Table 2 Special primer pairs for amplifying human SSTR-2, SSTR-3, and SSTR-5 subtypes by RT-PCR
Primers | Sequences of primers | Size of PCR products (bp) |
|---|---|---|
SSTR-2 | ||
Sense | 5′-CATCATTGGGTTGTGTGCA-3′ (162 to 181) | 256 |
Antisense | 5′-GCTCGATGCTCATGACTGTC-3′ (399 to 418) | |
SSTR-3 | ||
Sense | 5′-TGGCATTGGGTTGTTCATG-3′ (263 to 282) | 435 |
Antisense | 5′-CTTCACCAGGGTTGTGTGTAG-3′ (675 to 696) | |
SSTR-5 | ||
Sense | 5′-GTTCATTGGGTTGTGTGCG-3′ (169 to 187) | 101 |
Antisense | 5′-CAACATTGGGTTGTGTGCC-3′ (262 to 381) | |
Positive control | ||
F-1 | 5′-CATCATTGGGTTGTGTGCA-3′ | 462 |
R-1 | 5′-CATCATTGGGTTGTGTGCA-3′ | |