Figure 5
Modulation of sensitivity to Gem by ERK1/2-Bcl-2 signal. BXPC-3 cells were transfected with siRNA duplexes (200 nM) specific to Bcl-2 or scrambled control in complete medium for 24 h before treatment with gemcitabine (0–25 uM) for 72 h. (A) Western blot assay for Bcl-2 expression in BXPC-3 cells; (B) cell apoptosis was determined using the ELISA assay; and (C) cell apoptosis was determined using the TUNEL assay. The results (mean ± SEM) were from four independent experiments. *P < 0.05.