Figure 4
Evaluation of cell apoptosis by PD98059 pretreatment using ELISA technique in pancreatic cancer BXPC-3 cells. Sensitization of pancreatic tumor BXPC-3 cells to PD98059- and/or gemcitabine-induced apoptotic cell death by ELISA assay (A) and TUNEL assay (B) after 24 h of pretreatment with 25 μM PD98059, 0–25 μM gemcitabine, or combination of PD98059 and 0–25 μM gemcitabine for 72 h. Increased apoptotic response was evident in the combination treatment group of the cells relative to untreated control or individual treatment groups; *P < 0.05, statistical significance.