Figure 4

Effects of AS clusterin ODN and/or gmcitabine treatment on clusterin expression in BxPC-3 cells. In A, cells were treated with various concentrations of gmcitabine for 48 h, total protein was then extracted and analyzed for clusterin and GAPDH levels by western blotting. In B, cells were treated with 10 u M gmcitabine for indicated intervals, total protein was then extracted, and clusterin and GAPDH levels were analyzed by western blotting. In C, cells were treated daily with 500 nM AS clusterin ODN or a 2-base clusterin MM control ODN for48 h. After a 24-h exposure to 10 u M gmcitabine, total rotein was then extracted, and clusterin and GAPDH levels were analyzed by western blotting.