Figure 3

Sequence-specific and dose-dependent inhibition of clusterin expression by AS clusterin ODN in BxPc-3 cells. In A, BxPc-3 cells were treated daily with various concentrations of AS cluserin ODN or a 2-base clusterin MM ODN as a control for 2 days, total cell protein was extracted from culture cells, and clusterin and GAPDH levels were analyzed by western blotting. In B, quantitative analysis of clusterin protein levels after normalization to GAPDH levels in BxPc-3 cells after treatment with various concentrations of AS clusterin ODN or MM control ODN was performed using laser densitometry. Each point represents the mean of triplicate analyses with SD. *, differs from controls (P < 0.01) by Student's t test.