Figure 2From: Clusterin confers gmcitabine resistance in pancreatic cancerExpression level of clusterin and gmcitabine IC50 of pancreatic cancer cell lines. A, RT-PCR analysis clusterin mRNA in 5 pancreatic cancer cell lines (PT45-P1, T3M4, BxPc-3, Capan-1 and PancTu-1). B, Western blot analysis of 5 pancreatic cancer cell lines (PT45-P1, T3M4, BxPc-3, Capan-1 and PancTu-1) using a monoclonal antibody specific for the clusterin α chain. As a protein loading control, the same blot was incubated with an anti-GAPDH monoclonal antibody. C, Correlation between clusterin expression and gmcitabine resistance. Linear regression analysis showed a statistically significant relationship between clusterin expression and gmcitabine IC50 (R2, P = 0.001).Back to article page