Effects of Ad/shBcl-xL on the in vivo sensitivity of LoVo cells to irradiation. A. Protein samples extracted from tumors at day 7 or 42 were determined using Western blot analysis for Bcl-xL expression levels. GAPDH was used as a loading control. B. Immunohistochemistry was performed to detect the expression of Bcl-xL protein in tissues of LoVo tumors treated with PBS, Ad/shcontrol or Ad/shBcl-xL at day 7 or 42. C. Proliferation of tumors in the mice injected with LoVo cells treated with PBS, Ad/shcontrol or Ad/shBcl-xL. D. Dectection of average tumor size at day 42 after the inoculation of LoVo cells treated with PBS, Ad/shcontrol or Ad/shBcl-xL. All experiments were performed in triplicate (n = 3). *P < 0.05 vs mock.