Figure 6

CCL25-induced stromelysin expression by ovarian cancer cells. Cells were tested for their ability to express stromelysins (MMP-3, MMP-10, and MMP-11) mRNA and protein. OVCAR-3 and CAOV-3 cells were cultured for 24 hours alone, with 100 ng/mL of CCL25, or 100 ng/mL of CCL25 + 1 μg/mL of monoclonal antibodies against CCR9. Total RNA was isolated and quantitative RT-PCR analysis was performed for mRNA expression of stromelysins (upper panel) and transcript copies are presented relative to actual copies of 18 S rRNA. Active stromelysins were quantified by Fluorokine and Biotrak assays in conditioned media (lower panel). MMP expression below the detectable limit of the RT-PCR is designated as below detection (BD). Asterisk(s) indicate statistical differences (p < 0.01) between untreated and CCL25-treated OvCa cells.