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Figure 5 | World Journal of Surgical Oncology

Figure 5

From: CCL25-CCR9 interaction modulates ovarian cancer cell migration, metalloproteinase expression, and invasion

Figure 5

CCL25-induced gelatinase expression by ovarian cancer cells. Cells were tested for their ability to express gelatinases (MMP-2 and MMP-9) mRNA and protein. OVCAR-3 and CAOV-3 cells were cultured for 24 hours alone, with 100 ng/mL of CCL25, or 100 ng/mL of CCL25 + 1 μg/mL of monoclonal antibodies against CCR9. Total RNA was isolated and quantitative RT-PCR analysis was performed for mRNA expression of gelatinases (upper panel) and transcript copies are presented relative to actual copies of 18 S rRNA. Active gelatinases in conditioned media were quantified by Fluorokine and Biotrak assays (lower panel). MMP expression below the detectable limit of the RT-PCR is designated as below detection (BD). Asterisk(s) indicate statistical differences (p < 0.01) between untreated and CCL25-treated OvCa cells.

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