Figure 3

CCR9-mediated ovarian cancer cell migration and invasion. (A) OVCAR-3 and CAOV-3 cells were tested for their ability to migrate toward chemotactic gradients of CCL25. Cells were co-cultured with 1.0 μg/mL mouse anti-CCR9 antibody during migration assays using 100 ng/mL of CCL25. (B) OVCAR-3 and CAOV-3 cells were also tested for their ability to invade or translocate across Matrigel™ matrix in response to 100 ng/mL of CCL25. Cells were co-cultured with 1.0 μg/mL monoclonal antibodies against CCR9 during invasion assays using 100 ng/mL of CCL25. The number of cells ± SEM that migrated or invaded is shown with asterisk(s) that indicate significant differences (p < 0.01) between no additions and chemokine-induced cells.