Figure 2

CCR9 mRNA and cell surface protein expressed by ovarian cancer cells. Total RNA was isolated from OVCAR-3 and CAOV-3 cell lines and normal primary ovary tissue. Quantitative RT-PCR analysis of CCR9 mRNA expression was performed in triplicate. The copies of transcripts are expressed relative to actual copies of 18 S rRNA ± SEM. OVCAR-3 and CAOV-3 cells were stained with PE-conjugated isotype control antibodies (solid histogram) or PE-conjugated anti-CCR9 monoclonal antibodies (open histogram) and quantified by flow cytometry. The mean fluorescent intensities of PE-positive cells are shown. Asterisk(s) indicate statistical significance (p < 0.01) between normal tissue and OvCa cells.