Table 1 Clone, dilution, and source of antibodies used for the immunohistochemical analysis in this study
ANTIBODY | CLONE | DILUTION | SOURCE |
|---|---|---|---|
EMA | Clone E29 | 1/800 | Dako |
Ki-67 | Clone MIB-1 | 1/50 | Immunotech |
PCNA | Clone PC-10 | N/A | Ventana |
Bcl-2 | Clone 124 | 1/20 | Dako |
P53 | Clone DO-7 | 1/50 | Dako |
Bad | 48 | 1/20 | BD (BioSciences) |
Mcl-1 | 38G3 | 1/500 | Novacastra |
Bcl-x | NC1 | 1/20 | Novacastra |
Mdm2 | IB10 | 1/40 | Novacastra |
Bak | polyclonal | 1/20 | Dako |
Cyclin D1 | polyclonal | 1/50 | Dako |
Bax | polyclonal | 1/50 | Dako |
Ki67 | Clone MIB-1 | 1/50 | Immunotech |
P53 | Clone DO-7 | 1/50 | Dako |
P16 | F-12 | 1/100 | Santa-Cruz |
P21 | EA10 | 1/10 | Oncogene |
P27 | SXS3G8 | 1/20 | Dako |
Cyclin D1 | P2D11F11 | Predilute | Ventana |