Figure 3From: Does Doxycycline work in synergy with cisplatin and oxaliplatin in colorectal cancer?Quantitative presentation of the real-time PCR of caspase 3 gene expression in HT 29 cells. The HT 29 colorectal cancer cells were treated with cisplatin and oxaliplatin for 24 hours with or without doxycycline. The caspase 3 gene expression was measured in 2 steps; reverse transcription and real time PCR. (CON = Control, Dox = DOX = Doxycycline treatment for 24 hours, CIS1; 4; 24 = Cisplatin treatment for 1, 4 and 24 hours respectively, CD 1; 4; 24 = Cisplatin and Doxycycline treatment for 1, 4 and 24 hours respectively, OXA 1; 4; 24 = Oxaliplatin treatment for 1, 4 and 24 hours respectively, OD 1; 4; 24 = Oxaliplatin and Doxycycline treatment for 1, 4 and 24 hours respectively, **, * – Statistically significant difference). The data are depicted as means of three experiments ± standard deviation.Back to article page