Figure 2

Gel electrophoresis of Caspase 3 RT PCR products in HT 29 cells treated with cisplatin or oxaliplatin with or without doxycycline. The upper part reveals qualitative assessment of caspase 3 gene expression while lower part shows quantitative assessment in different cell samples. (NC = Negative control, Con = CON = Positive control, Dox = DOX = Doxycycline treatment for 24 hours, C(CIS)1; C4; C24 = Cisplatin treatment for 1, 4 and 24 hours respectively, cd(CD)1; cd4; cd24 = Cisplatin and Doxycycline treatment for 1, 4 and 24 hours respectively, O(OXA)1; O4; O24 = Oxaliplatin treatment for 1, 4 and 24 hours respectively, od(OD)1; od4; 0d24 = Oxaliplatin and Doxycycline treatment for 1, 4 and 24 hours respectively, * – Statistically significant difference). The data are depicted as means of three experiments ± standard deviation.