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Table 2 Sources, dilution and pretreatment of antibodies used

From: A case of matrix-producing carcinoma of the breast

Antibody Clone Manufacturer Dilution Pretreatment
ER 1D5 DakoCytomation, USA 1:50 boiling (pH9.0, 40 min)
PgR PgR636 DakoCytomation, USA 1:800 boiling (pH9.0, 40 min)
HER2 DakoCytomation, USA Prediluted (Hercep test) boiling (pH6.0, 40 min)  
CK5/6 D5/16B4 DakoCytomation, Denmark 1:100 autoclave (pH6.0, 10 min)
CK14 LL002 NeoMarkers, USA 1:100 autoclave (pH6.0, 10 min)
CK17 E3 DakoCytomation, Denmark 1:40 autoclave (pH6.0, 10 min)
EGFR 2-18C9 DakoCytomation, USA Prediluted (pharmDX kit) proteinase K (room temperature, 5 min)
AE1/AE3 AE/AE3 DakoCytomation, Denmark 1:50 pronase (37 C, 15 min)
CK7 OV-TL12/30 DakoCytomation, Denmark 1:50 pronase (37 C, 15 min)
CK8 35βH11 DakoCytomation, USA 1:50 pronase (37 C, 15 min)
CK19 RCK108 DakoCytomation, Denmark 1:50 autoclave (pH6.0, 10 min)
α-SMA 1A4 DakoCytomation, Denmark 1:100  
P63 4A4 DakoCytomation, Denmark 1:50 autoclave (pH6.0, 10 min)
GFAP 6F2 DakoCytomation, Denmark 1:100