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Table 2 Sources, dilution and pretreatment of antibodies used

From: A case of matrix-producing carcinoma of the breast

Antibody

Clone

Manufacturer

Dilution

Pretreatment

ER

1D5

DakoCytomation, USA

1:50

boiling (pH9.0, 40 min)

PgR

PgR636

DakoCytomation, USA

1:800

boiling (pH9.0, 40 min)

HER2

DakoCytomation, USA

Prediluted (Hercep test)

boiling (pH6.0, 40 min)

 

CK5/6

D5/16B4

DakoCytomation, Denmark

1:100

autoclave (pH6.0, 10 min)

CK14

LL002

NeoMarkers, USA

1:100

autoclave (pH6.0, 10 min)

CK17

E3

DakoCytomation, Denmark

1:40

autoclave (pH6.0, 10 min)

EGFR

2-18C9

DakoCytomation, USA

Prediluted (pharmDX kit)

proteinase K (room temperature, 5 min)

AE1/AE3

AE/AE3

DakoCytomation, Denmark

1:50

pronase (37 C, 15 min)

CK7

OV-TL12/30

DakoCytomation, Denmark

1:50

pronase (37 C, 15 min)

CK8

35βH11

DakoCytomation, USA

1:50

pronase (37 C, 15 min)

CK19

RCK108

DakoCytomation, Denmark

1:50

autoclave (pH6.0, 10 min)

α-SMA

1A4

DakoCytomation, Denmark

1:100

 

P63

4A4

DakoCytomation, Denmark

1:50

autoclave (pH6.0, 10 min)

GFAP

6F2

DakoCytomation, Denmark

1:100

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