Figure 4

Cell death was determined by flow cytometry of annexin V- and 7-AAD-stained cells at 72 hours post-transfection with shLDH-A (RNAi) or siControl (NC). The histogram (right) represents the average percentage (±SD) of dead cells. The number of dead cells treated with shLDH-A (RNAi) compared with the control group (NC) has a P value of <0.05 using Student’s t test. 7-ADD, 7-aminoactinomycin D; RNAi, RNA interference; SD, standard deviation; sh, small hairpin.