Figure 4

EGFR expression was suppressed by RBM5 overexpression in A549 xenograft tumors. A549 xenografts were established and the RBM5 gene was delivered into xenografts by attenuated Salmonella. The mice were sacrificed on day 42. (A) Tumor sizes in nude mice in different groups. (B) Comparision of A549 xenografts taken out from different groups. (C) H&E staining of tumors of nude mice in different groups (40×). (D) RT-PCR analysis of the expression of relative RBM5 mRNA in the xenograft tumors. (E) RT-PCR analysis of the expression of relative EGFR mRNA in the xenograft tumors. (F) Western blot analysis of RBM5 and EGF R protein expression in the xenograft tumors. (G) Quantification of protein expression relative to β-actin. β-actin was used as an internal control. CON, non-transfected control group; NC(OE), negative control group; OE, RBM5 overexpression group. Data shown are means ± SD of three separate experiments. *P <0.05 and **P <0.01 indicate significant difference as compared to the negative control.