Figure 2

Overexpression of RBM5 in A549 cells by lentivirus-mediated gene expression system. A549 cells were infected with lentiviral vector LV-RBM5. Three days after infection, the relative RBM5 mRNA and protein expression were determined by RT-qPCR and Western blot analysis respectively. β-actin was used as an internal control. A, RT-qPCR analysis for RBM5 mRNA in different groups. B, Western blot analysis for RBM5 protein in different groups. C, Quantification of RBM5 protein levels relative to β-actin; CON, non-transfected control group; NC(OE), negative control group; OE, RBM5 overexpression group. Data shown are means ± SD of three separate experiments. **P <0.01 indicates significant difference as compared to the control.