Figure 2

Microscopic examination of the accessory mammary gland. (A) The cancer cells have infiltrated around a mammary duct (arrowhead) (hematoxylin and eosin stain, original magnification × 10). (B) Cytokeratin stain enhances the cancer cells and the mammary duct. The mammary duct is adjacent to the cancer cells (arrowhead) (cytokeratin AE1/AE3 immunostain, original magnification × 10). (C) The cancer cells have pale to slightly eosinophilic cytoplasm and a nucleus with pale chromatin (hematoxylin and eosin stain, original magnification × 20). (D) The cancer cells are negative for E-cadherin (E-cadherin immunostain, original magnification × 20).