Inhibition of cell proliferation and migration of PC-3 cell in response to aquapore 5 (AQP5) silencing. (A) The efficacy of AQP5 siRNA for knockdown of AQP5 mRNA was confirmed by PCR. (B) The efficacy of AQP5 siRNA for knockdown of AQP5 protein was confirmed by Western blotting. (C) Quantification of mRNA. (D) Quantification of protein. (E) Effect of AQP5 silencing on the viability of PC-3 cells. (F) Effects of AQP5 silencing on the invasion of PC-3 cells. Data from at least three independent experiments with duplicate determinations are expressed as means ± SEM. *P < 0.05 was considered statistically significant. (G): Columns in the graph are the diagram of the count analysis.