Figure 5
sCLU regulates the cisplatin (DDP)-induced cytotoxicity in the osteosarcoma (OS) cells. (A) The KH OS or sCLU stably transfected KH OS cells (KH OS/sCLU) were treated with 0.01 to 10 μM/ mL DPP for 72 hours in the presence or absence of 10 mM PD98059. Cell viability was determined using a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The results were obtained from three independent experiments. Versus KHOS/sCLU, *P < 0.01; versus KH OS, **P < 0.01. (Χ2 test). (B) The sCLU-shRNA stably transfected U-2 OS cells (U-2 OS/sCLU-shRNA-1 and U-2 OS/sCLU-shRNA-2) were treated with 0.01 to 10 μM/mL DPP for 72 hours in the presence or absence of 5 μM MEK1. Cell viability was determined using an MTT assay. The results (mean ± SEM) were obtained from three independent experiments. sCLU shRNA1/2 groups versus MEK1 groups, *P < 0.01; sCLU shRNA1/2 groups versus U-2 OS control groups, **P < 0.01 (Χ2 test).