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Figure 1 | World Journal of Surgical Oncology

Figure 1

From: Secreted clusterin (sCLU) regulates cell proliferation and chemosensitivity to cisplatin by modulating ERK1/2 signals in human osteosarcoma cells

Figure 1

Clusterin (CLU) expression positively correlates with pERK1/2 expression. (A) Western blot analysis was performed to determine basal expression of CLU and pERK1/2 expression in indicated OS cell lines. (B) Western blot showing 90% reduction in CLU levels in two U-2 OS subclones stably transfected with sCLU-specific shRNA. Untreated and vector alone-transfected U-2 OS cells show high basal levels of CLU expression. (C) Western blot showing 95% increase in CLU levels in KH OS/sCLU clones stably transfected with sCLU. Untreated and vector alone-transfected KH OS cells show low basal levels of CLU expression. The membranes were stripped and reprobed with anti-β-actin antibody to ensure even loading of proteins in each lane. Results shown are from representative experiments repeated at least twice with similar findings. Versus control, *P < 0.05.

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