Figure 1

GLI mRNA expression in stage II-IV lung adenocarcinoma patients. The levels of GLI1, GLI2, and GLI3 mRNA expression were measured by quantitative real-time polymerase chain reaction in stage II-IV lung adenocarcinoma samples. The three GLI mRNA were detected in all 102 samples, except three patients who were negative for GLI2. Wide variations were observed in GLI1 and GLI3 mRNA levels. The top 15% of patients with the highest GLI expression were defined as the higher expression group in each category. Each mRNA value was expressed as a ratio to GAPDH mRNA (internal control).