Figure 2

NCTD caused apoptotic death in U87 (A, C) and C6 (B, D) cells. Following 10 h of cell treatments, cells were collected and stained with Annexin V/PI followed by FACS analysis. Representative FACS analysis scatter-grams of Annexin V/PI stained 0, 10, 30, and 50 μM NCTD treatment showed four different cell populations marked as: double negative (unstained) cells showing live cell population (lower left), Annexin V positive and PI negative stained cells showing early apoptosis (lower right), Annexin V/PI double-stained cells showing late apoptosis (upper right), and finally PI positive and Annexin V negative stained cells showing dead cells (upper left). Apoptosis was defined as Annexin V staining positive. *P <0.05 compared to the control group.