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Table 2 Antibodies used in immunostaining in immunohistological analyses.

From: An inflammatory myofibroblastic tumor exhibiting immunoreactivity to KIT: a case report focusing on a diagnostic pitfall

Antigen Clone Dilution Source Pretreatment
SMA 1A4 Prediluted Nichirei None
Desmin D33 Prediluted Nichirei Microwave (10 min)
Pan-cytokeratin AE1/AE3 Prediluted Nichirei Protease
KIT Polyclonal 1:25 DAKO Microwave (10 min)
ALK 5A4 1:50 Nichirei Boiling (30 min)*
PDGFR Polyclonal 1:50 Santa Cruz Boiling (60 min)
DOG1 SP31 1:50 Nichirei Boiling (60 min)
IgG EPR4421 1:50 Epitomics Boiling (30 min)
IgG4 HP6025 1:50 Nichirei Boiling (60 min)
  1. * The iAEP method was required to obtain the signals. ALK, anaplastic lymphoma kinase; IgG, immunoglobulin G; PDGFR, platelet-derived growth factor receptor.