Table 2 Antibodies used in immunostaining in immunohistological analyses.
Antigen | Clone | Dilution | Source | Pretreatment |
|---|---|---|---|---|
SMA | 1A4 | Prediluted | Nichirei | None |
Desmin | D33 | Prediluted | Nichirei | Microwave (10 min) |
Pan-cytokeratin | AE1/AE3 | Prediluted | Nichirei | Protease |
KIT | Polyclonal | 1:25 | DAKO | Microwave (10 min) |
ALK | 5A4 | 1:50 | Nichirei | Boiling (30 min)* |
PDGFR | Polyclonal | 1:50 | Santa Cruz | Boiling (60 min) |
DOG1 | SP31 | 1:50 | Nichirei | Boiling (60 min) |
IgG | EPR4421 | 1:50 | Epitomics | Boiling (30 min) |
IgG4 | HP6025 | 1:50 | Nichirei | Boiling (60 min) |