Figure 3
From: Differential expression of p42.3 in low- and high-grade gliomas
Generation and identification of the p42.3 antibody. (A) p42.3 was expressed in Escherichia coli and purified by nickel nitrilotriacetic acid agarose. (B) Western blot analysis showed that two sets of rabbit antiserum could specifically bind the purified protein of p42.3. (C) Mass spectrometry analysis confirmed that the sequence of purified protein corresponded to that of p42.3. (D) Mascot database (Matrix Science, Boston, MA, USA) search results.