Figure 3

sCLU silencing inhibits invasion of human Hep-2 cells in vitro. A. The bar shows the number of migratory cells transfected with sCLU siRNA and control siRNA. At bottom is the quantification of migratory cells in control siRNA and sCLU siRNA transfected Hep-2 cells. The experiment was done in triplicate and the value obtained from control siRNA transfected cells was set as 100% migration. B. A wound was scratched in a confluent cell layer of Hep-2 cells. Cells were either transfected with control siRNA (control) or sCLU siRNA for 24 h. The percentage of coverage after 24 h of wound healing is depicted in the graphs for Hep-2 cells. Knockdown of sCLU significantly reduces wound healing in Hep-2 cells. Significance was determined with a Student's t-test vs control, ^*P <0.05. sCLU, Clusterin; siRNA, small interference RNA.