Figure 1From: Clusterin silencing inhibits proliferation and reduces invasion in human laryngeal squamous carcinoma cellsEffect of sCLU-siRNA transfection on mRNA and protein expressions of sCLU. A. Western immunoblot analysis of sCLU expression in Hep-2 cells transfected with sCLU-siRNA. Blots were re-probed with β-actin antibody to analyze the equal loading of proteins. B. Semiquantitative RT-PCR analysis shows mRNA expression of sCLU in Hep-2 cells transfected with sCLU-siRNA. Scrambled siRNA was used as a control in parallel. β-actin was used as an internal control. This is a representative example with mean densitometric values from triplicate blots. *, P <0.05 versus control siRNA transfectants. sCLU, Clusterin; siRNA, small interference RNA.Back to article page