Hemangioma vascular endothelial cell (HVEC) culture and identification. (A) The HVECs were derived from tissues resected during the surgery of children aged 59 days who had strawberry hemangioma on the abdominal surface. The tissues were cut into small pieces, inoculated into culture flasks, and maintained in culture medium. After 3 days, the endothelial cells migrated quickly from the tissue pieces. One week later, some of the endothelial cells became confluent (×100). (B) The HVECs were identified with human vascular endothelial cells factor VIII related antigen (VIII-R Ag) immunostaining (×200). (C) The HVECs were also analyzed with VEGFR2 flow cytometry. The percentage of HVECs in cells that we isolated and subcultured was 59.1%.