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Table 1 Experimental conditions of immunohistochemistry assays

From: The relevance of the intrinsic subtype to the clinicopathological features and biomarkers in Japanese breast cancer patients

Antibody (clone) Dilution Source Criteria for positive/negative
ER : Antibody (ID5) ×100 Dako Positive: Stained cells >10%
PR : Antibody (PgR636) ×500 Dako Positive: Stained cells >10%
HER2 (HercepTest) Ready to use Dako HercepTest criteria
EGFR (EGFR pharmDx kit) Ready to use Dako EGFR pharmDx kit criteria
CK5/6 (D5/16 B4) ×100 Dako Positive: Stained cells >10%
PTEN (6H2.1) 1:100 Dako - No staining
    + Decreased staining intensity
    ++ Equal or increased staining intensity
IGF-1R (C-20) 1:50 Santa Cruz Score 0, 1+, 2+, 3+ using HercepTest criteria
Bcl-2 (124) ×50 Dako Positive: Stained cells >10%
c-Kit (polyclonal; CD117) ×500 Dako Positive: Stained cells >10%
c-Met (C-28, polyclonal) 1:100 IBL Positive: Stained cells >10%
HIF-1α (H1alpha67) ×100 Novus Biologicals Positive: Stained cells >10%
PDGFRA (C20, polyclonal) 1:200 Thermo Positive: Stained cells >10%
Survivin (12C4) ×200 Novus Biologicals Positive: Stained cells >20%
VEGFR2 (polyclonal; Flk-1(A-3)) ×100 Santa Cruz Positive: Stained cells >10%
VEGF-A (VG1) Ready to use Thermo Positive: Stained cells >10%
  1. ER: estrogen receptor, PR: progesterone receptor, HER2: human epidermal growth factor receptor type 2, EGFR: epidermal growth factor receptor, CK5/6: cytokeratin 5 or 6, PTEN: phosphatase and tensin homolog deleted on chromosome 10, IGF-1R: insulin-like growth factor-1 receptor, Bcl-2: B-cell lymphoma 2, c-Met: hepatocyte growth factor receptor, HIF-1α: hypoxia-inducible factor 1-alpha, PDGFRA: alpha-type platelet-derived growth factor receptor, VEGFR2: vascular endothelial growth factor receptor 2, VEGF-A: vascular endothelial growth factor A.
  2. NOTE. Dako: Tokyo, Japan. Santa Cruz: Tokyo, Japan. IBL: Gunma, Japan. Novus Biologicals: Littleton, Colorado, USA. Thermo: Waltham, Massachusetts, USA.