Table 1 Identified citrullinated proteins in cultured tumour cell lines using 2-D western blotting
From: Investigating citrullinated proteins in tumour cell lines
Spots in cell lines | MALDI-TOF/TOF MS analysis | Identified proteins |
|---|---|---|
ECA 3a | Mass: 61187 Score: 133 Expect: 1.2e-08 Matches: 17 | 60 kDa heat shock protein (HSP60) |
H292 6a | Mass: 54454 Score: 104 Expect: 9.2e-06 Matches: 11 | Protein disulfide isomerase (PDI) |
HeLa 1a | Mass: 60813 Score: 76 Expect: 0.054 Matches: 6 | 60 kDa heat shock protein (HSP60) |
HEPG2 2a | Mass: 50240 Score: 155 Expect: 7.3e-11 Matches: 22 | Alpha-enolase (ENO1) |
HEPG2 3a | Mass: 36628 Score: 102 Expect: 1.5e-05 Matches: 12 | T cell receptor beta chain (TCRb) |
HEPG2 6a | Mass: 53738 Score: 149 Expect: 2.9e-10 Matches: 18 | Vimentin (VIME) |
Lovo 1a | Mass: 48083 Score: 105 Expect: 7.3e-06 Matches: 9 | Mitochondrial ATP synthase, H + transporting F1 complex beta subunit |
MCF-7 3a | Mass: 53774 Score: 130 Expect: 2.3e-08 Matches: 12 | Cytokeratin 8 (KRT8) |
PANC-1 1a | Mass: 53738 Score: 140 Expect: 2.3e-09 Matches: 17 | Vimentin (VIME) |
PANC-1 4a | Mass: 47481 Score: 120 Expect: 2.3e-07 Matches: 13 | Alpha-enolase (ENO1) |
PANC-1 4a | Mass: 36628 Score: 79 Expect: 0.027 Matches: 9 | T cell receptor beta chain (TCRb) |
SGC 1a | Mass: 50177 Score: 70 Expect: 0.026 Matches: 10 | Tubulin beta (TUBB) |
SGC 2a | Mass: 36628 Score: 113 Expect: 1.2e-06 Matches: 11 | T cell receptor beta chain (TCRb) |
SGC 3a | Mass: 47481 Score: 172 Expect: 1.5e-12 Matches: 15 | Alpha-enolase (ENO1) |
SKOV 3a | Mass: 61187 Score: 103 Expect: 1.2e-05 Matches: 14 | 60 kDa heat shock protein (HSP60) |