Figure 1From: Investigating citrullinated proteins in tumour cell linesTwo-dimensional polyacrylamide gel electrophoresis and the parallel 2-D western blotting of total proteins from various tumour cell lines. The total proteins extracted from ECA, H292, HeLa, HEPG2, Lovo, MCF-7, PANC-1, SGC, and SKOV3 cells were separated using two identical 2-DE gels. The tumour cell proteins on one gel were trans-blotted onto a PVDF membrane and probed with an anti-citrulline antibody. The protein spots in the same position as those recognised by the parallel 2-D WB were selected and identified by MALDI-TOF MS.Back to article page