Figure 2

H&E, immunohistochemistry and TUNEL assay. (A) Immunohistochemistry staining for RBM5 (400×). (B) H&E staining (200×). ‘↑’ indicates cancer nest. (C) RBM5-induced apoptosis shown by TUNEL staining (400×). Tissue sections were treated with terminal deoxynucleotidyl transferase to incorporate fluorescein-deoxyuridine 5'-triphosphate (dUTP) to nicked DNA of apoptotic cells. Apoptotic cells exhibiting a strong nuclear green fluorescence were detected by fluorescence microscopy. Adjacent light microscopic photos showed a representative area from each group showing equal cell density and fixation. RBM5, RNA-binding motif protein 5.