Figure 2

Effect of TNF-α on Bcl-2 expression. Lung cancer cells were treated with 20 ng/ml TNF-α. (A) Time course of Bcl-2 mRNA induction by TNF-α in A549 lung cancer cells. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was loaded as control. (B) Western blot analysis of Bcl-2 protein expression in A549 cells followed TNF-α treatment for different times. (C) Wedelolactone-pretreated A549 lung cancer cells were transfected with SLUG or the control scrambled siRNA, and then treated with TNF-α for 48 h. SLUG, Bcl-2 and p-P65 expression was analyzed by western blot. (D) Wedelolactone-pretreated A549 lung cancer cells were transfected with SLUG or the control scrambled siRNA, and then treated with TNF-α for 24 h. SLUG, Bcl-2 and p-P65 expression was analyzed by reverse transcription PCR.