Figure 1

Effect of TNF-α on nuclear factor kappa B (NF-κB) and SLUG expression. Lung cancer cells were treated with 20 ng/ml TNF-α. NF-κB transactivation function was determined by electrophoretic mobility shift assay. SLUG mRNA and protein at different time points after treatment were analyzed by reverse transcription PCR(RT-PCR) and western blotting, respectively. (A) NF-κB transactivation function was determined by electrophoretic mobility shift assay at different time points after TNF-α treatment. (B) Time course of SLUG mRNA induction by TNF-α in A549 lung cancer cells. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was a control. (C) Time course of SLUG mRNA induction with or without TNF-α/wedelolactone in A549 lung cancer cells. GAPDH was a control. (D) Time course of SLUG protein induction by TNF-α in A549 cells. Phospho-p65 and β-actin were also analyzed. (E) Time course of SLUG and phospho-p65 protein induction with or without TNF-α/wedelolactone in A549 lung cancer cells.