Figure 3

4′,6-Diamidino-2-phenylindole staining of BT325 (A) and U251 (B) cells treated with sorafenib (2.5 μM) plus vitamin K1 (VK1) (50 μM). Cells were treated with sorafenib, VK1 or their combination for 24 h. The cells treated with the combination of the agents showed morphological changes of apoptosis, including a condensed and fragmented nuclear structure and decreased cell size (original magnification 400×).