Figure 4

Mutational analysis of K-ras codon 12 by PCR-RFLP (digestion with BSTNI restriction endonuclease). PCR product size: 157 bp, Normal K-ras allele: 114 bp, Mutant K-ras allele: 143 bp. Heterozygous mutant case: 143 bp and 114 bp. Μ = molecular weight marker pUC Mix 8. Lane 1 = Undigested PCR product (157 bp). Lanes 7-9 = Mutant samples (143 bp and 114 bp). Lanes 2-6 & 10 = Normal samples (114 bp).