Figure 1

Amiloride down-regulated the production of urokinase (u-PA) in MKN45 gastric cancer cells. The MKN45 cells were added in the dishes at the amount of 2 × 10⁶ cells per dish. After incubation for 6 h, 12 h or 24 h, cells were treated with amiloride in concentrations of 0.01 mM, 0.1 mM or 1 mM, and RPMI 1640 with 10% FBS was used as a control. (A) Expression of u-PA mRNA. (B) The level of u-PA. (C) u-PA activity. Con, control. Data are means ± SD, ^*P < 0.05 vs. Con.