Table 1 Optimal conditions for RNA in situ hybridization
Tumor-related gene | Digestion time (min) | Incubation time (h) | Incubation temperature (°C) | Chromogenic time (min) |
|---|---|---|---|---|
MYC | 20 | 42 | 41.5 | 30 |
CCND1 | 20 | 36 | 45 | 50 |
TP53 | 30 | 44 | 48 | 110 |
UVRAG | 30 | 38 | 18.5 | 60 |
RB1 | 25 | 42 | 21 | 45 |
PTEN | 20 | 40 | 19.5 | 45 |
PTCH1 | 22 | 40 | 23 | 40 |
BRCA1 | 24 | 39 | 22 | 40 |
BRCA2 | 24 | 39 | 23 | 35 |
FHIT | 20 | 44 | 24 | 40 |
BECN1 | 24 | 46 | 20.5 | 25 |
BCL10 | 25 | 40 | 19.5 | 90 |
APC | 25 | 37 | 20 | 80 |
CD82 | 25 | 40 | 29 | 35 |
NME1-NME2 | 22 | 46 | 20.5 | 50 |