Figure 1

RNA in situ hybridization of TP53 expression for normal tissue adjacent to cancerous tissue and normal tissue adjacent to benign tissue. (A-D) show the NTAC from the thyroid, breast, liver and colon cancer samples, respectively. (A′-D′) show the NTAB. Unlike in malignant RISH, there is no clonal patch, but there is a scattered TP53-positive cell distribution, indicated by the arrow. The positive cell number and density were visualized on a Zeiss-Axiophot DM HT microscope (Zeiss-Axiophot, Oberkochen, Germany) and captured with an attached camera linked to a computer plus EZ-HYB software. NTAB: normal tissue adjacent to benign tissue; NTAC: normal tissue adjacent to cancerous tissue; RISH: RNA in situ hybridization.