Figure 2

Overexpression of RBM5 in PC-3 cells. PC-3 cells were transfected with pcDNA3.1 or pcDNA3.1-RBM5 plasmids for 48h. RT-PCR and western blot were performed respectively to determine the RBM5 mRNA (A) and protein levels (C). Data shown are means±S.D. of three separate experiments (C for mRNA and D for protein). * indicates significant difference as compared to the mock control and EV (P <0.01).