Skip to main content

Table 1 The primers of p53 gene and the condition of amplification

From: Malignant epithelioid angiomyolipoma of the kidney with pulmonary metastases and p53 gene mutation

Exon

Amplified length (bp)

Primer sequence (5′-3′)

Condition of amplification

5

183

TACTCCCTGCCCCTCAACAAG

94°C, 1 minute; 55°C, 1 minute; 72°C,

  

ATCGCTATCTGAGCAGCGCTC

1 minute; 48 cycles

6

114

GGTCTGGCCCCTCCTCAGCAT

94°C, 1 minute; 63°C, 1 minute;72°C,

  

CTCAGGCGGCTCATAGGGCAC

1 minute; 50 cycles

7

111

GTTGGCTCTGACTGTACCACC

94°C, 1 minute; 55°C, 1 minute;72°C,

  

CCTGGAGTCTTCCAGTGTGAT

1 minute; 48 cycles

8

135

TGGTAATCTACTGGGACTGAA TCGCTTAGTGCTCCCTGGGGG

94°C, 1 minute; 55°C, 1 minute; 72°C, 1 minute; 48 cycles