Inhibition of p28GANK expression represses the proliferation of U251 cell in vitro and in vivo. (A) The expression of p28GANK in Si-U251 cells was markedly lower compared with the controls (Con-251 and U251) on Western blot analysis. (B): Growth curves of U251, Con-U251 and Si-U251 cells by MTT assays. The results presented were the mean of three replicates. (C) Comparison of glioma cell tumorigenicity in mice. The left flank was injected with Si-U251 cells, and the right flank was injected with Con-U251 cells. (D) Tumor volumes formed by si-U251 were significantly smaller than Con-U251 cells. P < 0.05.