Figure 6

Assessment of tumor-preferable distribution of the bacteria. Tissue samples from the lung tumor xenografts, liver, lung, spleen, heart and kidney of three tumor-bearing mice treated with bacteria carrying vector control were collected for bacterial distribution analysis. Tissue (100 mg) from either tumors or each organ in 3 ml of PBS was homogenized well, and the homogenized tissue was plated onto Luria-Bertani agar. The result was observed after 14 h. (A) Representative images of the plates planted with different tissues at day 3 (72 h) and day 7 (148 h) after bacterial injection. T, tumor xenograft; Li, liver; Lu, lung; S, spleen; H, heart; K, kidney. (B) and (C) Quantitative analysis of the bacterial counts by CFU per gram of tissue at day 3 (B) and day 7 (C) after bacterial injection. *Significant difference in tumor xenograft group as compared to other group (p < 0.05).