Figure 4
From: The tumor suppressor gene RBM5 inhibits lung adenocarcinoma cell growth and induces apoptosis
Effect of RBM5 overexpression on apoptosis in A549 cells. A549 cells were harvested at 48 h after transfection. PS flip to the outer nuclear membrane was monitored by staining cells with FITC-conjugated Annexin V, which binds to the PS and PI (A). Data are presented as a flow cytomter dot plot. Q1, live cells; Q2, early apoptotic cells; Q3, late apoptotic cells; Q4, necrosis cells. (B) The representative data of FCM with Annexin V and PI staining for detecting apoptotic cells are shown in control and RBM5 transfected groups. *Significant difference as compared to the control (p < 0.05).