Effect of RBM5 overexpression on A549 cells proliferation. A549 cells were transfected with pcDNA3.1 or pcDNA3.1-RBM5 plasmids for up to 24 h and 48 h. Afterward, cell growth was observed under microscopy (A) and evaluated by MTT assay (B). The data are presented as the ratio compared to the control (control = 100%). Data shown are means ± SD of three separate experiments. * p < 0.05, ** p < 0.01 indicate significant difference as compared to the control.