Figure 4

The effect of HGF inhibition on cell growth and migratory abilities of glioma cells. a MTT assays were performed in the U87MG glioma cell line. Cells were cultured in a 96-well plate and transfected with HGF siRNA or control siRNA for 24, 48 and 72 h points with an average of five independent experiments in each group (*P <0.05 versus control cells; one-way ANOVA). b Optical microscopic images of in vitro wound healing at 0, 12, 24 and 48 h after the creation of wounds. HGF siRNA-transfected cells displayed significantly slower wound closure at all time points compared with control siRNA-transfected cells.