Figure 3

HGF siRNA inhibited tumor cell-derived HGF expression. a Immunofluorescence assay showed that tumor cell-derived HGF protein expression was significantly decreased in U87MG glioma cells with HGF siRNA transfection (bottom) when compared with control siRNA transfected cells (middle) and mock (top) (immunofluorescence staining with original magnification, ×400). Western blot assay (b) and RT-PCR (c) exhibited that HGF protein and mRNA expression levels were decreased in the cells with HGF siRNA transfection.