Semi-quantitative RT-PCR analysis of selected genes. Total RNA isolated from 4 primary tumor tissue samples and 4 corresponding metastasis lymph node tissue samples were used as a template for RT-PCR using the gene-specific primers described in Table 2. RT-PCR showing the down-regulation of (A) RPL29, (B) CD98,(C)L6 and (D)TSH. (E)GAPDH was used as an internal reference. Pi, primary tumor; Mi, metastasis tumor (i refer to the number of sample).