Fig. 6

Single-cell analysis between TFP and TFN tumors. A–E UMAP visualization of 8469 cells among five PC samples. F Heatmap of differentially expressed genes (rows) between cells classified into inferred seven cell subsets. Bars on the top of the heatmap indicated the cell type corresponding to those of A with selected genes indicated. G Distribution of immune-related cells subpopulations in TFP vs TFN tumors. H–K UMAP of tumor cells identified by the Copycat method. L Histogram showed the prediction results of PC samples by 5-ERG-mRPs. The row represented the times that a sample was diagnosed as TFP or TFN sampler by a random method. *p < 0.05, **p < 0.01, ***p < 0.001. M Violin plots showing expression of ERG, TNPO1, EXTL2, DPP4, and ANG in among four samples